WS13.01 The cystic fibrosis Home Sputum-Induction Trial (CF-HomeSpIT - ISRCTN86523335) to evaluate home sputum-induction and early morning saliva sampling in children with cystic fibrosis

Objectives: In CF patients on modulator therapy, sputum volume and pathogen density is significantly reduced, yet complete eradication uncommon. This mandates a reassessment of current approaches to infection surveillance. Early morning saliva (emSal) home sputum-induction (hSI) may be enriched with lower airway pathogens from undisturbed muco-ciliary clearance overnight present an attractive novel proposition. The CF-Home Sputum Induction Trial (CF-HomeSpIT) non-blinded prospective internally-controlled interventional trial comparing emSal hSI sampling matched same day samples clinic (cSI), saliva(cSal) cough swab(cCS) samples. Methods: CF-HomeSpIT will recruit 100 opened in September 2022. All children at the Children’s Hospital for Wales, aged 5–18 yrs, already using Hypertonic saline (HS) are eligible recruitment. Home packs provided by post consent instructions delivered phone (www.uhwchildren.com/CF-HomeSpIT). Table(abstract: WS13.01).Patient IDEarly Morning Saliva (emSal)Home Sputum-Induction (hSI)Clinic Cough Swab (cCS)Clinic (cSal)Clinic (cSI)5S. aureusS. aureus6S. marcescens10Enterobacter11P. aeruginosaP. aeruginosa14P. aeruginosa, H. influenzaeH. influenzaeP. influenzae Open table new tab EmSal obtained 3 rounds standardised manoeuvre [COUGH, HUFF, COUGH, CLEAR THROAT, SPIT]. For hSI, use HS their normal physiotherapy regimen, SPIT, whenever they feel rattle. Matched taken physiotherapist standard protocols. Results: interim analysis, first 14 identified 6 pathogens. 4/6 (66%) were isolated emSal, 3/6 (50%) 2/6 (33%) cCS, cSal, 5/6 (87%) cSI. combined. A second analysis (with estimated ~40 patients) presented conference. Conclusion: Results suggest home-sampling possible, comparable yield compared supervised sampling. novel, quick, practical approach that warrants further evaluation.